US Patent for Maize inbred 2ZZSB1121 Patent (Patent # 11,589,535 issued The versatile properties of Cre recombinase make it ideal for use in many genetic engineering strategies. Missirlis PI, Smailus DE, Holt RA. Can be used for injection into fertilized zygotes, Cre activates gene of interest by removing neoR and stop sequence; used for making transgenic mice. Therefore, researchers often use transgenic mice expressing CreERt2 recombinase induced by tamoxifen administration, under the control of a promoter of a gene that marks the specific cell type of interest, with a Cre-dependent fluorescent protein reporter. The Cre/lox-site-specic recombination system derived from bacteriophage P1 is known to be appropriate for genome modication [23-26], and its mechanism of action has been clearly described [27-29]. Marth and Rajewsky reported in 1994 that Cre-lox recombination could be used for conditional gene targeting. In addition, sequence excision can happen in trans instead of a in cis cassette exchange event. a CAG promoter) and a loxP flanked stop cassette, ensuring the expression of the transgene is Cre-recombinase dependent and the reporter sequence. A number of conserved site-specific recombination systems have been described in both prokaryotic and eukaryotic organisms. 2 D ). The circular molecules contain site-specific recombination sequences and allow transformation of host cells with DNA comprising only selected sequences of interest.
PDF Cre/lox-mediated chromosomal integration of biosynthetic gene clusters The https:// ensures that you are connecting to the The Cre/lox system can also be used to produce strains in which a transgene is either inducible or expressed only in certain tissues. Heteromeric . [15][16] As of February 2019, CreLox recombination is a powerful tool and is used in transgenic animal modeling to link genotypes to phenotypes.[12][17][18]. 14.1k views Gene knockout samuel kwatia 52.1k views Knock out mice Priya Nanda The Lox or loxP sites (locus of X over P1) are the palindromic sites recognized by cre-recombinase. Cre-lox ? What do I need to know about the customs and importation process for my country? the recombination sites in a size-independent manner. This tool is suitable for deleting antibiotic resistance genes, but above all it allows conditional knockouts that can be induced at specific times in the cell type of choice. Now customize the name of a clipboard to store your clips. In this edition, were going to take a look at a very interesting tool that can be used for creating (excuse the pun)specific, targeted DNA modificationsin transgenic animals, embryonic stem cells, and/or tissue-specific cell types:Cre-lox recombination.
Cre-Lox recombination - Columbia University Postdoc Society Blog An official website of the United States government. A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination. It is one of the most powerful tools in the mouse geneticists toolbox. Well, it enables them to generate tissue-specific and inducible knockouts and thereby have exquisite control over the location and timing of gene expression important stuff when deleting a certain gene everywhere or during development leads to an embryonic lethal phenotype.
Usually loxP sites come in pairs for genetic manipulation. Receive the latest news, hot plasmids, discounts and more. Expressed in fibroblasts, TFP and Cre-ERT2 - Tamoxifen inducible. 8bp region (spacer region), and two Models thus obtained are more likely to mimic the physiological situation. Mol Urol. FOIA CRY2-CreN and mCherry coexpression; Light inducible; Use with plasmid 26889. mouse), and another with the loxP sites (the floxed mouse) are created, and crossed to. This has been largely due to the simplicity of its use and its adaptability to address diverse biological questions. The present invention relates generally to transgenic plants. Engineered lox variants which differ on the spacer region tend to have varied but generally lower recombination efficiency compared to wildtype loxP, presumably through affecting the formation and resolution of recombination intermediate.[25]. In tissues with no cre gene the target gene with be present and function normally. These constructs allow for Cre-regulated gene expression. Cre-Lox recombination involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase.
17.
Once tamoxifen is introduced, it is metabolized into 4-hydroxytamoxifen, which then binds to the ER and results in the translocation of the CreER into the nucleus, where it is then able to cleave the lox sites. Cre Recombinase Cyclization recombinase (Cre) is one of the tyrosine site-specific recombinases, which is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites). Primary * One unit is defined as the amount of enzyme necessary to produce maximal site-specific recombination of 0.25 g pLox2+ control DNA in 30 minutes at 37C in a total reaction volume of 50 l. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally. Conditional gene inactivation can be achieved with the Cre/ lox system in which the bacteriophage Cre recombinase promotes site-specific recombination at compatible loxP sites engineered in a gene of interest ( Sauer and Henderson, 1988 ). The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators.
How Does Cre, Flp, Dre Recombinase System Work? If you are looking for the right Cre-expressing strain for your experiments, you can search for strains at the JAX Cre Repository or through the Cre Portal at MGI. Chief among these is Cre recombinase, discovered in the P1 bacteriophage and named as such because it c auses re combination (Cre) between specific DNA sequences referred to as loxP (locus of crossover (x) in P1) sites, each of which are 34 base pairs in length 1. <The exam for Jae Lee's sessions> Please provide a ' brief and concise ' answer to each question. Cre-lox recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control gene expression, especially in in vivo models, and new Cre-lox based technologies are still being developed today. For example, mating the transgenic strain in Figure 1 to a strain that expresses Cre recombinase in mammary tissue produces double transgenic offspring that express theKras oncogene only in the mammary glands (Figure 5). Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage.The enzyme uses a topoisomerase I-like mechanism to carry out site specific recombination events. An attP site on the phage exchanges segments with an attB site on the bacterial DNA. The site is secure. Cre recombinase proteins bind to the first and last 13 bp regions of a lox site forming a dimer. By continuing to use this site, you agree to the use of cookies. Using an in vivo phagemid system to identify non-compatible loxP sequences. Increasing the length of DNA leads to decreased efficiency of Cre/lox recombination possibly through regulating the dynamics of the reaction. A separate recombinase recognizes and binds to each of two recombination sites on two different DNA molecules or within the same DNA strand. place. Research mouse models that incorporate the Cre/loxsystem typically involve generating double,triple, quadruple, or evenn-tuple(is that really a word?) To generate mice that are heterozygous for aloxP-flanked allele and hemizygous/heterozygous for thecretransgene, mate a homozygousloxP-flanked mouse of interest to a cre transgenic mouse strain (see Figure 1 below).
Cre-Lox Models: 6 Facts You May Not Know - ingenious targeting laboratory Upon Cre driven recombination, the stop cassette is excised, allowing reporter genes to express specifically in cells in which the Cre expression is being driven by the cell-specific marker promoter. This is also the catalytic site of the enzyme.
Effective production of kojic acid in engineered Aspergillus niger 2001 Aug 17;311(3):453-9. doi: 10.1006/jmbi.2001.4888. To do so, one mouse with the Cre recombinase (the Cre When there are FEBS Lett. Editing, Cloning The products of the recombination event depend on the relative orientation of these asymmetric sequences. Site-specific recombination (SSR) involves specific sites for the catalyzing action of special enzymes called recombinases. Removal of selectable markers from the genome by Cre-lox recombination is an elegant and efficient way to circumvent this problem and is therefore widely used in plants, mouse cell lines, yeast, etc.[1]. Cre/lox switchingexpression system Currently, mostwidely used site-specific DNA recombination system system[18, 19]. Cre activates gene of interest, using 4 mutated lox sites, Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors, Multibow uses a binary code in which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination to maximize label diversity, Rosa26 targeting vector, Cre dependent tdtomato expression. a) The chromosomal copy of the target gene replaces the target gene flanked by loxP sites. To create a conditional knock-out mouse, one needs the following systems: the Cre-loxP, and recombinase, and loxP sites. You will have to adapt it to the genotypes you want to produce and the genetic backgrounds and characteristics of theloxP-flanked andcrestrains you use.
US Patent for Maize hybrid X00R808 Patent (Patent # 11,589,542 issued 18 Raj-Nandgaon ( C. G. ). Looks like youve clipped this slide to already. [2], Cre-Lox recombination is a special type of site-specific recombination developed by Dr. Brian Sauer and patented by DuPont that operated in both mitotic and non-mitotic cells, and was initially used in activating gene expression in mammalian cell lines. Cre-Lox recombination is a site-specific recombinase technology widely used to carry out deletions, insertions, translocations and inversions in the DNA of cells. 2. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through CreloxPmediated gene targeting. [30] Importantly, sometimes fluorescent reporters can be activated in the absence of tamoxifen, due to leakage of a few Cre recombinase molecules into the nucleus which, in combination with very sensitive reporters, results in unintended cell labelling. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. cre-lox and cre recombinases in Mouse Genome Informatics (MGI): Module 2.
Cre/ lox -mediated recombination in Arabidopsis - Academia.edu Many other proteins apart from the recombinase are involved in regulating the reaction. This includes the introduction of FRT sites that may be used in the FLP/FRT system and/or Lox sites that may be used in the Cre/loxP system. The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics. Embodiments include the seeds of soybean 4826008, the plants of soybean 4826008, to plant parts of soybean 4826008, and methods for producing a soybean plant produced by crossing soybean 4826008 with itself or with another soybean variety. [7] Combining these advances in collaboration, the laboratories of Drs. Cre-lox Systems Cre-lox (floxed) systems for gene deletion ( click here for non-floxed and earlier floxed tagging vectors) General Comments The baseline reference for this system is CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei (2008) Scahill MD, Pastar I & Cross GAM. Linker, Adaptor, Homopolymeric Tailing & Terminal Transferase, Transcriptional and post transcriptional regulation of gene expression, Molecular biology of the gene ch 13 rna splicing part1, Alternative splicing : mechanism and regulation, POST TRANSCRIPTIONAL MODIFICATIONS IN EUKARYOTES, Nepalgunj Medical College and Teaching Hospital, govt .science college ,tumkur,( govt.estab), artificial or synthetic transcription factor for regulation of gene expression, Enzyme Kinetics and thermodynamic analysis, Chromatin, Organization macromolecule complex, eukaryotic translation machinery by kk sahu, Easy-handling carbon nanotubes decorated poly(arylene ether nitrile).pdf. At the given specific site on the DNA, the hydroxyl group of the tyrosine in the recombinase attacks a phosphate group in the DNA backbone using a direct transesterification mechanism. The gene of interest lies In this method, the marker gene gets removed due to the induc- Structure of the Cre-loxA complex evaluation of unintended effects, ignoring the fact that conven- besides the lox sites. Activate your 30 day free trialto continue reading. The .gov means its official. Activate your 30 day free trialto unlock unlimited reading. Addgene has a wide variety of Cre recombinase plasmids. A key advantage of the system is that Cre acts independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety of experiments. Comparative mitochondrial zygomycetes: bacterial-like Rnase P RNAs, mobile el No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. You can read the details below. Clipboard, Search History, and several other advanced features are temporarily unavailable. Bookshelf Such conditional lineage tracing has proved to be extremely useful to efficiently and specifically identify vascular smooth muscle cells (VSMCs) and VSMC-derived cells and has been used to test effects on VSMC and VSMC-derived cells in vivo.[32][33][34][35][36][37]. With genomic tumor testing, a negative result is not always definitive. How can I track requests for my plasmids? Hepatic Nampt Deficiency Aggravates Dyslipidemia and Fatty Liver in High Fat Diet Fed Mice. The schematic below shows the three types of rearrangements: inversion, deletion and translocation. PubMed, Gu, H., Zou, Y.R., and Rajewsky, K. 1993. One of my colleagues, Andy, has written an awesome blog post on howCre recombinasecan produce a phenotype on its own. government site. Unauthorized use of these marks is strictly prohibited. 2001 Sep 21;505(3):467-73. doi: 10.1016/s0014-5793(01)02806-x. I. Recombination between loxP sites. Rafik Karaman. Thus, these are site-specific, occurring only at the respective att sites. How can I be notified when a plasmid from a specific lab or paper is available? Aranda M, Kanellopoulou C, Christ N, Peitz M, Rajewsky K, Drge P. J Mol Biol. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 2ZZSB1121 through backcross conversion and/or transformation, and to the .
Gene activation via Cre/lox-mediated excision in cowpea (Vigna About 25% of the offspring from this mating will be homozygous for theloxPflanked allele but will have nocretransgene. Cell Culture Media, Introduction to the Cre-Lox Recombination System, AAALAC-accredited and OLAW assured animal centers, Specific-pathogen-free (SPF) animal health status exceeds requirements of most facilities, Top-tier customer support: free consultations and quotes.
Cre Recombinase - California Lutheran University The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. 5 Phosphate ends are usually the substrates for this reaction, thus extended 3 regions remain. PubMed, CRISPR Expression Systems and Delivery Methods. You will earn 10 point for each question correctly answered (140 points for 14 . Methods for producing a maize plant by crossing hybrid maize variety X00R808 with another maize plant are disclosed. Furthermore, recombination systems such as Cre-lox are widely used, mostly in eukaryotes. A few of the most common uses are listed below.
In vitro method to create circular molecules for use in transformation Simply put, the Cre/loxsystem rocks.
Cre-Lox Recombination - Science topic - ResearchGate Axin2+ Peribiliary Glands in the Periampullary - Gastroenterology PubMedPMID:8513499. hbspt.cta._relativeUrls=true;hbspt.cta.load(306096, '3d466a06-620b-4f16-8841-5197527ea7a8', {"useNewLoader":"true","region":"na1"}); Topics: P1 phage DNA on the other hand, exists as a plasmid in the host. These developments have led to a widespread use of conditional mutagenesis in biomedical research, spanning many disciplines in which it becomes a powerful platform for determining gene function in specific cell types and at specific developmental times. The Cre/ lox system is one of the most powerful and versatile tools developed for mouse genetics. This dimer then binds to a dimer on another lox site to form a tetramer.
Lecture 11 - Lectures For UG-5 - studyres.com The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The mechanism of Cre recombination requires two lox sites be brought together by looping the intervening DNA.
PPT PowerPoint Presentation (Figure 1), recombination between Cre- loxP cre loxP GFP cre GFP cre cre cre 2022 Dec 14;15:1043018. doi: 10.3389/fnmol.2022.1043018. When such a reporter strain is mated to a Cre-expressing strain, it produces offspring in which the visible marker is expressed only in tissues with Cre activity (Figure 8). called the FLP (pronounced 'flip') protein. * Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project The FLP-FRT technology can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for two separate recombination events to be controlled in parallel. Furthermore, the Cre ERT gene is knocked in to the Axin2 locus in Axin2-Cre ERT mice,
Cre-Lox: A Tool for Removal of Marker Genes to Make GM Foods Safe This gene-inactivating mutation will be passed on, creating a constitutive knockout line for the gene. 10. eCollection 2023 Jan. Wang N, Lv L, Huang X, Shi M, Dai Y, Wei Y, Xu B, Fu C, Huang H, Shi H, Liu Y, Hu X, Qin D. Front Mol Neurosci. In some cases a genomic variant can be present but not detected by the, With November designated as Family Health History month, clinicians are sure to have seen lots of messaging, The microbiome plays a vital role in our health and wellbeing. These will be your experimental mice. NIH Blueprint for Neuroscience Research has created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community.
Cre-loxP-Mediated Recombination: General Principles and - PubMed Jae Lee-Exam.docx - <The exam for Jae Lee's sessions> Would you like email updates of new search results?
Cn201110179613.2- By accepting, you agree to the updated privacy policy. As such, the Cre lox system has been used in a wide variety of eukaryotes, including plants. Cre recombinase removes the stop sequence, so the transgene is expressed only in the cells where Cre is active (see Figure 4 below). Please note that if you list up a lengthy description of irrelevant points (which happens when you do not exactly know the real answer), it will reduce your score even if you provide a correct answer or answers. the loxP sites will delete the gene
Tetracycline-inducible Expression Systems New - Tetrad analysis, positive and negative interference, mapping through somatic Chromosome walking jumping transposon tagging map based cloning, Conversion of fibroblasts to retinal cells by transcription (final). orientation of the two loxP, one can create a gene inversion, translocation, or deletion. This system can be implemented without inserting any extra supporting proteins or sequences. Cre (shown at left) is a bacteriophage P1 member of the integrase family, catalyzing site-specific recombination between two, 34-base pair lox DNA sequences. 3. Nov 2, 2015. We applied the Cre/lox recombinase-mediated cassette exchange (RMCE) system to Aedes aegypti, the vector of dengue, chikungunya, and Zika viruses. See more Base pair. Cre-Lox recombination is commonly used to circumvent embryonic lethality caused by systemic inactivation of many genes. Fill out our Suggest a Plasmid form or e-mail [emailprotected] to help us improve this resource! We've encountered a problem, please try again. The Cre-binding site in DNA is called the LoxP sequence that consists of 34 nucleotides DNA sequence made up of two inverted repeats separated by a spacer. An important application of the Cre-lox system is excision of selectable markers in gene replacement. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Another factor is the length of DNA between the lox pair. Clipping is a handy way to collect important slides you want to go back to later. Sternberg, N. and Hamilton, D. 1981. Digvijay Autonomous P. G. College Thank you for your participation! The Cre- lox system consists of the 38.5-kDa Cre recombinase and the 34-bp lox site. Depending on the Introduction
Cre-lox recombination
Cre-lox system- Cre recombinase , loxP site
FLP-FRT recombination
FLP-FRT system- FLP recombinase , FRT site
Mechanism of Cre-lox and FLP-FRT recombination
Binding
Synapsis , cleavage and strand exchange
Three type of arrangement
Inversion
Translocation/ Insersion
Deletion
Application of Cre-lox and FLP-FRT recombination
Disadvantage of FLP-FRT
Advantage and disadvantage of Cre-lox
Conclusion
References. [10] Tsien and his colleagues demonstrated Cre-mediated recombination can occur in the post-mitotic pyramidal neurons in the adult mouse forebrain.[11]. Cre-Lox recombination is a sophisticated site-specific recombinase technology that allows DNA modification to be targeted to a specific cell type or to be triggered by a specific external stimulus. FEBS Lett.
Bacterial genome editing by coupling Cre- lox and CRISPR-Cas9 - PLOS Explanation: The Cre recombinase protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP. Figure Recombination involve pairing between complementary strands of the two parental DNAs. found in the yeast and based on the yeast two-micron plasmid. This website uses cookies to ensure you get the best experience. Thus, the orientation, and location of the loxP sites are important determinants of This reaction links the recombinase protein to the DNA via a phospho-tyrosine linkage. In vivo, the function of Cre recombinase is to circularize the P1 genome during infection and maintain the genome in the monomeric state for cell division. Depending on the construct, Cre may activate or repress gene expression. Mate these mice back to the homozygousloxP-flanked mice (see Figure 2 below). Notice that only one generation of breeding is required (Hooray!). 1. Energy is not compromised, since the protein-DNA linkage makes up for the loss of the phosphodiester bond, which occurred during cleavage. Cre-lox in vivo/vitro . If the two loxP sites are in the same orientation, the floxed sequence (sequence flanked by two loxP sites) is excised; however if the two loxP sites are in the opposite orientation, the floxed sequence is inverted. To verify that Cre recombinase expression does not contribute to a phenotype of interest, I suggest using thecrestrain itself (without anyloxP-flanked alleles) as a control (Figure 3B). We've encountered a problem, please try again. aegypti. Unable to load your collection due to an error, Unable to load your delegates due to an error. This recombinase comes from the P1 bacteriophage. Have you used the Cre-lox system in your research?
Cre-lox-regulated conditional RNA interference from transgenes A double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated, resulting in the reciprocal crossover event. recombinase binding elements
SOYBEAN CULTIVAR 08130343 - M.S. Technologies, L.L.C. In the lysogenic cycle the phage genome replicates with the rest of the bacterial genome and is transmitted to daughter cells at each subsequent cell division. The loxP site (34bp) has a central Orban, P.C., Chui, D., and Marth, J.D. The obvious difference between the two systems is that the recombinase (Cre and Flp) has different optimal reaction temperatures. It is also proposed that rolling circle replication followed by recombination will allow the plasmid to increase its copy number when certain regulators (repA) are limiting. Expressed in chondrocytes, mCherry and Cre-ERT2 - Tamoxifen inducible; Targeting vector for Pax7, Venus and Cre-ERT2 with MCS for inserting promoter, Emerald and Cre-ERT2 with MCS for inserting promoter, TFP and Cre-ERT2 with MCS for inserting promoter, tdTomato and Cre-ERT2 with MCS for inserting promoter, Venus, Cre-ERT2, targeting vector with MCS for homology arms, Emerald, Cre-ERT2, targeting vector with MCS for homology arms, TFP, Cre-ERT2, targeting vector with MCS for homology arms, tdTomato, Cre-ERT2, targeting vector with MCS for homology arms, GAG-Cre fusion; for the production of VLPs loaded with CRE protein, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable linker) - Tamoxifen inducible, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 linker) - Tamoxifen inducible, iCre-ERT2 (PAPGSTMA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PA N-terminus) - Tamoxifen inducible, CreLite - Red light-inducible Cre; N terminus Cre fused to PIF6, CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta, CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit), CreLite; Tol2 destination vector with mTagBFP2, CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector, CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector, Cre recombinase split with Vivid photodimers, Cre-ERT2 - Tamoxifen inducible; AAV donor vector, iCre-ERT2 (PAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible, N-terminal Cre component split with Vivid photodimers, C-terminal Cre component split with Vivid photodimers, Split Cre fused to nuclear localized wild-type VVD, Retinal ganglion cell-specific expression of Cre, Cre activates shRNA expression, removal of EGFP; See also similar plasmids, Cre turns off shRNA expression, removal of EGFP; See also similar plasmids, Cre activates gene of interest by removing Stop sequences; Puro selection. [13][14] To date, NIH Blueprint for Neuroscience Research Cre projects have created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community. The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics.
Cre-Lox Recombination - an overview | ScienceDirect Topics In general, these systems use one or more proteins and act on unique asymmetric DNA sequences. And, it can be used to turn transgene expression on or off, track individual cells or cell lineages (as in theBrainboworConfettimice), generateinversionsortranslocations, and report gene expression.
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